LIPOSOME-ENTRAPPED SUPEROXIDE-DISMUTASE REDUCES CEREBRAL INFARCTION IN CEREBRAL-ISCHEMIA IN RATS

被引：237

作者：

IMAIZUMI, S ^{[1
]}

WOOLWORTH, V ^{[1
]}

FISHMAN, RA ^{[1
]}

CHAN, PH ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT NEUROL,CNS INJURY & EDEMA RES CTR,BOX 0114,SAN FRANCISCO,CA 94143

来源：

STROKE | 1990年 / 21卷 / 09期

关键词：

Brain edema; Cerebral infarction; Rats; Superoxide dismutase;

D O I：

10.1161/01.STR.21.9.1312

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We studied the role of Superoxide radicals in the pathogenesis of ischemic brain injury using a model of focal cerebral ischemia in 102 rats and liposome-entrapped CuZn-superoxide dismutase, which can penetrate the blood-brain barrier and cell membranes efficiently. The bolus intravenous administration of 25,000 units of liposome-entrapped CuZn-superoxide dismutase elevated Superoxide dismutase activities in the blood and brain 1, 2, 8, and 24 hours later as well as in the ischemic hemisphere and contralateral cortex. Determined 24 hours after right middle cerebral and bilateral common carotid artery occlusion by the lack of staining for mitochondrial dehydrogenase activity with 2,3,5-triphenyltetrazolium chloride, infarct sizes were reduced by 33%, 25%, and 18% in the anterior, middle, and posterior brain slices, respectively, by treatment with liposome-entrapped CuZn-superoxide dismutase. Our data demonstrate that Superoxide radicals are important determinants of infarct size following focal cerebral ischemia and that liposome-entrapped CuZn-superoxide dismutase may have pharmacologic value for the treatment of focal cerebral ischemic injury.

引用

页码：1312 / 1317

页数：6

共 35 条

[1] HYPOGLYCEMIC BRAIN INJURY - PHOSPHOLIPIDS, FREE FATTY-ACIDS, AND CYCLIC-NUCLEOTIDES IN THE CEREBELLUM OF THE RAT AFTER 30 AND 60 MINUTES OF SEVERE INSULIN-INDUCED HYPOGLYCEMIA [J].