EXPRESSION OF RAT PROLACTIN-LIKE PROTEIN-B IN DECIDUOMA OF PSEUDOPREGNANT RAT AND IN DECIDUA DURING EARLY-PREGNANCY

被引:37
作者
CROZE, F
KENNEDY, TG
SCHROEDTER, IC
FRIESEN, HG
机构
[1] UNIV MANITOBA,FAC MED,DEPT PHYSIOL,WINNIPEG R3E 0W3,MANITOBA,CANADA
[2] UNIV WESTERN ONTARIO,DEPT OBSTET & GYNECOL,LONDON N6A 5A5,ONTARIO,CANADA
[3] UNIV WESTERN ONTARIO,DEPT PHYSIOL,LONDON N6A 5A5,ONTARIO,CANADA
关键词
D O I
10.1210/endo-127-6-2665
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The rat PRL-like protein-B (rPLP-B) complementary DNA (cDNA), originally cloned from a late term placental library, hybridizes to transcripts in the deciduoma tissue artificially produced in pseudopregnant rats. The expression of rPLP-B in deciduoma is first observed 48 h (pseudopregnancy day 7) after the deciduogenic stimulus and increases to a maximum by 96-120 h (pseudopregnancy day 9-10). In situ hybridization studies show that rPLP-B hybridizes specifically to the antimesometrial cells of deciduoma tissue in day 9 pseudopregnant rats and to the homologous cells of the decidua that surround the embryo-trophoblastic structure at day 9 of pregnancy. A temporal study on the expression of rPLP-B during pregnancy shows that rPLP-B is concomitantly expressed by the decidual cells of maternal origin and the cytotrophoblast of fetal origin around day 13 of pregnancy. © 1990 by The Endocrine Society.
引用
收藏
页码:2665 / 2672
页数:8
相关论文
共 24 条
[1]  
Gibori G., Rothchild I., Pepe G.J., Morishige W.K., Lam P., Luteotrophic action of decidual tissue in the rat, Endocrinology, 95, pp. 1113-1118, (1974)
[2]  
Jayatilak P.G., Glaser L.A., Basuray R., Kelly P.A., Gibori G., Identification and partial characterization of a prolactin-like hormone produced by rat decidual tissue, Proc Natl Acad Sci USA, 82, pp. 217-221, (1985)
[3]  
Duckworth M.L., Peden L.M., Friesen H.G., A third prolactinlike protein expressed by the developing rat placenta: Complementary deoxyribonucleic acid sequence and partial structure of the gene, Mol Endocrinol, 2, pp. 912-920, (1988)
[4]  
Duckworth M.L., Schroedter I.C., Friesen H.G., Cellular localization of rat placental lactogen II and rat prolactin-like proteins A and B by in situ hybridization, Placenta, 11, pp. 143-155, (1990)
[5]  
Kennedy T.G., Doktorcik P.E., Uterine decidualization in hypophysectomized- ovariectomized rats: Effects of pituitary hormones, Biol Reprod, 39, pp. 318-328, (1988)
[6]  
Kennedy T.G., Intrauterine infusion of prostaglandins and decidualization in rats with uteri differentially sensitized for the decidual cell reaction, Biol Reprod, 34, pp. 327-335, (1986)
[7]  
Chirgwin J.M., Przybyla A.E., Macdonald R.J., Rutter W.J., Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases, Biochemistry, 18, pp. 5294-5299, (1979)
[8]  
Lehrach H., Diamond D., Wozney J.M., Boedtker H., RNA molecular weight determinations by gel electrophoresis under de naturing conditions, a critical reexamination, Biochemistry, 16, pp. 4743-4751, (1977)
[9]  
Thomas P.S., Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose, Proc Natl Acad Sci USA, 77, pp. 5201-5205, (1980)
[10]  
Murphy L.J., Seneviratne C., Ballejo G., Croze F., Kennedy T.G., Identification and characterization of a rat decidual insulin-like growth factor binding protein complementary DNA, Mol Endocrinol, 4, pp. 329-336, (1990)