FREQUENCY OF RECIPROCAL TRANSLOCATIONS AND DICENTRICS INDUCED IN HUMAN BLOOD-LYMPHOCYTES BY X-IRRADIATION AS DETERMINED BY FLUORESCENCE IN-SITU HYBRIDIZATION

被引：67

作者：

NAKANO, M ^{[1
]}

NAKASHIMA, E ^{[1
]}

PAWEL, DJ ^{[1
]}

KODAMA, Y ^{[1
]}

AWA, A ^{[1
]}

机构：

[1] RADIAT EFFECTS RES FDN,DEPT STAT,HIROSHIMA 732,JAPAN

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1993年 / 64卷 / 05期

关键词：

D O I：

10.1080/09553009314551781

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study was designed to test the scoring efficiency of reciprocal translocations and dicentrics induced by X-irradiation in vitro using the fluorescence in situ hybridization (FISH) technique. An excess was found in the frequencies of reciprocal translocations relative to those of dicentrics by measurement with FISH at the first cell division after irradiation (translocation:dicentric almost-equal-to 60:40). However, when the same metaphases were also evaluated sequentially by a conventional staining method, the ratio of about 50:50 was restored. This was due in part to misclassification of certain dicentrics as. reciprocal translocations by the FISH technique.

引用

页码：565 / 569

页数：5

共 8 条

[1]

AWA A A, 1978, Journal of Radiation Research, V19, P126, DOI 10.1269/jrr.19.126

[2] RAPID METAPHASE AND INTERPHASE DETECTION OF RADIATION-INDUCED CHROMOSOME-ABERRATIONS IN HUMAN-LYMPHOCYTES BY CHROMOSOMAL SUPPRESSION INSITU HYBRIDIZATION [J].