PROPERTIES AND AMINO-ACID-SEQUENCE OF HUWENTOXIN-I, A NEUROTOXIN PURIFIED FROM THE VENOM OF THE CHINESE BIRD SPIDER SELENOCOSMIA-HUWENA

被引：76

作者：

LIANG, SP ^{[1
]}

ZHANG, DY ^{[1
]}

PAN, X ^{[1
]}

CHEN, Q ^{[1
]}

ZHOU, PA ^{[1
]}

机构：

[1] BEIJING UNIV,NATL LAB BIOMEMBRANE & MEMBRANE BIOTECHNOL,BEIJING 100871,PEOPLES R CHINA

来源：

TOXICON | 1993年 / 31卷 / 08期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/0041-0101(93)90256-I

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

By means of reverse phase and ion-exchange high performance liquid chromatography, a neurotoxic peptide named huwentoxin-I was purified from the venom of the Chinese bird spider Selenocosmia huwena. The intraperitoneal and intracisternal LD50 in mice of the toxin were 0.70 mg/kg and 9.40 mug/kg, respectively. This toxin at the concentration of 1 x 10(-5) g/ml can irreversibly block the neuromuscular transmission of the isolated mouse phrenic nerve-diaphragm preparation in 13.4 +/- 1.3 min (mean +/- S.D., n = 5). The isoelectric point is 8.95 determined by isoelectric focusing electrophoresis. It consists of 33 amino acids including 6 Cys and 6 Lys determined by amino acid analysis. The complete amino sequence of huwentoxin-I was determined. The N-terminal and C-terminal residues were Ala and Leu, respectively. The primary structure showed partial homology with that of mu-agatoxins from the funnel-web spider Agelenopsis aperta.

引用

页码：969 / 978

页数：10