SEQUENCE-ANALYSIS OF THE UPSTREAM REGION OF DHLB, THE GENE ENCODING HALOALKANOIC ACID DEHALOGENASE OF XANTHOBACTER-AUTOTROPHICUS GJ10

被引：14

作者：

VANDERPLOEG, J ^{[1
]}

JANSSEN, DB ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT BIOCHEM,9747 AG GRONINGEN,NETHERLANDS

来源：

BIODEGRADATION | 1995年 / 6卷 / 03期

关键词：

BIODEGRADATION; HALOACETATE; SIGMA(54)-DEPENDENT REGULATION;

D O I：

10.1007/BF00700465

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The DNA sequence upstream of the dhlB gene encoding the haloalkanoic acid dehalogenase of Xanthobacter autotrophicus GJ10 was determined and contained an open reading frame, designated dhlC, which encoded a protein with a significant similarity with the family of Na+-dependent symport proteins. The dhlC gene was subcloned under control of a T7 promoter, and found to encode a polypeptide of 45 kDa on SDS-PAGE. Upstream of dhlC, a -24/-12 promoter sequence was found. Further upstream, in the opposite direction of transcription, another open reading frame, designated dhlR,with homology with the family of sigma(54)-dependent transcriptional activator proteins was detected. The dhlR gene was cloned and expressed under the control of a T7 promoter and encoded a polypeptide of 51 kDa on SDS-PAGE. The genetic organization of the dhlB region suggested that the expression of dhlC and dhlB was controlled by the product of dhlR and sigma(54) which may explain the observed overexpression of the haloalkanoic acid dehalogenase under starvation conditions.

引用

页码：257 / 263

页数：7

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