RIBOSOMAL-RNA GENES AND THE B-CHROMOSOME OF BRACHYCOME-DICHROMOSOMATICA

被引：30

作者：

DONALD, TM ^{[1
]}

LEACH, CR ^{[1
]}

CLOUGH, A ^{[1
]}

TIMMIS, JN ^{[1
]}

机构：

[1] UNIV ADELAIDE,DEPT GENET,ADELAIDE,SA 5005,AUSTRALIA

来源：

HEREDITY | 1995年 / 74卷

基金：

澳大利亚研究理事会;

关键词：

B CHROMOSOMES; BRACHYCOME DICHROMOSOMATICA; IN SITU HYBRIDIZATION; NUCLEOLAR ORGANIZER; RIBOSOMAL-RNA GENES;

D O I：

10.1038/hdy.1995.77

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from OB and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.

引用

页码：556 / 561

页数：6

共 27 条

[1] BEWILDERING BS - AN IMPRESSION OF THE 1ST B-CHROMOSOME CONFERENCE [J].