ISEV position paper: extracellular vesicle RNA analysis and bioinformatics

被引：130

作者：

Hill, Andrew F. ^{[1
]}

Pegtel, D. Michiel ^{[2
]}

Lambertz, Ulrike ^{[3
]}

Leonardi, Tommaso ^{[4
,5
,6
]}

O'Driscoll, Lorraine ^{[7
]}

Pluchino, Stefano ^{[4
,5
]}

Ter-Ovanesyan, Dmitry ^{[8
]}

Nolte-'t Hoen, Esther N. M. ^{[9
]}

机构：

[1] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3010, Australia

[2] Vrije Univ Amsterdam, Med Ctr, Canc Ctr Amsterdam, Dept Pathol, Amsterdam, Netherlands

[3] Univ British Columbia, Dept Med, Div Infect Dis, Vancouver, BC, Canada

[4] Univ Cambridge, Dept Clin Neurosci, John van Geest Cambridge Ctr Brain Repair, Cambridge, England

[5] Univ Cambridge, Stem Cell Inst, Wellcome Trust Med Res Council, Cambridge, England

[6] European Bioinformat Inst, EMBL, Cambridge, England

[7] Trinity Coll Dublin, Sch Pharm & Pharmaceut Sci, Dublin, Ireland

[8] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[9] Univ Utrecht, Dept Biochem & Cell Biol, Fac Vet Med, Utrecht, Netherlands

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2013年 / 2卷 / 01期

基金：

英国惠康基金;

关键词：

extracellular vesicles; deep sequencing; evRNA; RNA; bioinformatics;

D O I：

10.3402/jev.v2i0.22859

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on "evRNA analysis and bioinformatics.'' Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis.

引用

页数：8

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