EXPRESSION OF PROCESSED CORE PROTEIN OF HEPATITIS-C VIRUS IN MAMMALIAN-CELLS

被引：137

作者：

HARADA, S

WATANABE, Y

TAKEUCHI, K

SUZUKI, T

KATAYAMA, T

TAKEBE, Y

SAITO, I

MIYAMURA, T

机构：

[1] NATL INST HLTH,DEPT MED ENTOMOL,SHINIAGAWA KU,TOKYO 141,JAPAN

[2] NATL INST HLTH,CTR AIDS,SHINAGAWA KU,TOKYO 141,JAPAN

[3] ST MARIANNA MED UNIV,SCH MED,MIYAMAE KU,KAWASAKI,KANAGAWA 213,JAPAN

[4] TOKYO NATL CHEST HOSP,TOKYO 204,JAPAN

[5] NATL INST HLTH,DEPT ENTEROVIRUSES,SHINAGAWA KU,TOKYO 141,JAPAN

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 06期

关键词：

D O I：

10.1128/JVI.65.6.3015-3021.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A structural protein of hepatitis C virus (HCV) was expressed in monkey COS cells under the control of an exogenous promoter, and a protein of 22 kDa was identified by immunoblot analysis. This protein (p22), which was produced by processing in COS cells, reacted specifically to sera of chronic hepatitis C patients, and its coding region was mapped at the most amino-terminal part of the HCV polyprotein. These results suggested that the p22 protein is the nucleocapsid (core) protein of HCV. Moreover, the assay detecting antibody to p22 was found to be useful for early diagnosis of HCV infection.

引用

页码：3015 / 3021

页数：7

共 24 条

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