USE OF NOVEL IMMOBILIZED BETA-GALACTOSIDASE REACTOR TO HYDROLYZE THE LACTOSE CONSTITUENT OF SKIM MILK .2. MULTIRESPONSE NONLINEAR-REGRESSION ANALYSIS OF KINETIC DATA

A novel chemical reactor, consisting of beta-galactosidase from Aspergillus oryzae immobilized on a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. Multiresponse nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a proposed enzymatic mechanism that includes the formation of oligosaccharides. HPLC methods were employed to monitor the concentrations of all species present in the effluent stream. For the experimental conditions used in this research, a rate expression that includes the inhibition effect of alpha-galactose is sufficient to model the reaction network.