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A QUANTITATIVE IMMUNOCYTOCHEMICAL METHOD FOR THE MEASUREMENT OF ISLET CELL CYTOPLASMIC ANTIBODIES

被引：3
作者：
MARSHALL, MO
HOYER, PE
机构：
[1] NOVO NORDISK AS,DEPT ENDOCRINOL & IMMUNOL,DK-2880 BAGSVAERD,DENMARK
[2] PANUM INST,DEPT MED ANAT A,COPENHAGEN,DENMARK
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 149卷 / 01期
关键词：
IMMUNOCYTOCHEMISTRY; ISLET CELL; CYTOPLASMIC; (ANTIBODY);
D O I：
10.1016/S0022-1759(12)80049-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A quantitative immunocytochemical method for the measurement of islet cell cytoplasmic antibodies has been developed. The method employs human or rat pancreas, a protein A-peroxidase/diaminobenzidine secondary antibody system and an independent measurement of islet total and exocrine mean integrated absorbance by scanning microdensitometry. Specific islet cell cytoplasmic antibody binding (islet total-exocrine mean integrated absorbance) was dependent on serum dilution and substrate reaction time. The detection limit was approximately 5 JDF units. Specific islet cell cytoplasmic antibody binding values with human and rat pancreas were similar. Specific islet cell cytoplasmic antibody binding (human pancreas) was greater (p < 0.001) in sera from patients with newly diagnosed insulin dependent diabetes mellitus (0.119 +/- 0.086, n = 29) compared to normal sera (0.003 +/- 0.008, n = 29). Thus, the method has been validated and may be useful for measuring the blocking effect of potential antigens on specific islet cell cytoplasmic antibody.
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页码：63 / 68
页数：6
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