EVALUATION OF A TEST BASED ON BACULOVIRUS-EXPRESSED GLYCOPROTEIN-G FOR DETECTION OF HERPES-SIMPLEX VIRUS TYPE-SPECIFIC ANTIBODIES

被引：80

作者：

SANCHEZMARTINEZ, D

SCHMID, DS

WHITTINGTON, W

BROWN, D

REEVES, WC

CHATTERJEE, S

WHITLEY, RJ

PELLETT, PE

机构：

[1] CTR DIS CONTROL,CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS,MAILSTOP G-18,1600 CLIFTON RD,ATLANTA,GA 30333

[2] CTR DIS CONTROL,CTR PREVENT SERV,DIV SEXUALLY TRANSMITTED DIS & HIV PREVENT,ATLANTA,GA 30333

[3] BIOKIT SA,DEPT MOLEC BIOL,BARCELONA,SPAIN

[4] UNIV ALABAMA,DEPT PEDIAT & MICROBIOL,BIRMINGHAM,AL 35294

来源：

JOURNAL OF INFECTIOUS DISEASES | 1991年 / 164卷 / 06期

关键词：

D O I：

10.1093/infdis/164.6.1196

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An immunoblot assay for discrimination of antibodies to herpes simplex virus (HSV) types 1 and 2 was devised using extracts of recombinant-baculovirus-infected insect cells expressing HSV-1 or -2 glycoprotein G (gG1 or gG2). The assay was evaluated by comparing its results with those obtained by using an immunodot assay based on gG immunopurified from HSV-1- and HSV-2-infected cells. Each of 110 human serum specimens was tested blindly and independently three times. At a serum dilution of 1:20, the maximum specificities were 96% and 100% and the maximum sensitivities were 100% and 92% for gG1 and gG2, respectively. Reproducibility was 99% among readers and 95% among individually tested samples of each specimen. Results obtained in two laboratories from a different set of 15 serum specimens were in complete agreement, indicating the assay is accurate and reproducible. The ease of antigen production should allow the test to become widely available.

引用

页码：1196 / 1199

页数：4

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