GENOMIC AND CDNA CLONING OF A NOVEL MOUSE LIPOXYGENASE GENE

被引：23

作者：

VANDIJK, KW ^{[1
]}

STEKETEE, K ^{[1
]}

HAVEKES, L ^{[1
]}

FRANTS, R ^{[1
]}

HOFKER, M ^{[1
]}

机构：

[1] GAUBIUS LAB,PG TNO,2301 CE LEIDEN,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1259卷 / 01期

关键词：

INFLAMMATION; ARACHIDONIC ACID; ATHEROSCLEROSIS; LIPOGENASE GENE; (MOUSE);

D O I：

10.1016/0005-2760(95)00158-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel 12- and 15-lipoxygenase related gene was isolated from a mouse strain 129 genomic phage library in a screen with a human 15-lipoxygenase cDNA probe. The complete genomic sequence revealed 14 exons and 13 introns covering 7.3 kb of DNA. The splice junctions were verified from the cDNA sequences of a series of overlapping RT-PCR products. This novel mouse lipoxygenase gene was found to contain an open reading frame encoding 662 amino acids. The cDNA sequence is 68% identical to the mouse leukocyte type 12-lipoxygenase and 65% identical to the mouse platelet type 12-lipoxygenase cDNA. Nucleotide identities to the human 15-lipoxygenase cDNA and the human platelet type 12-lipoxygenase cDNA are 70% and 67%, respectively. The deduced amino acid sequence is 60% identical to the mouse platelet and leukocyte type 12-lipoxygenase sequences and the human 15- and 12-lipoxygenase sequences. This novel mouse lipoxygenase gene was found to be transcribed predominantly in epidermal tissue and named Aloxe.

引用

页码：4 / 8

页数：5

共 18 条

[1]

ARAKAWA T, 1992, J BIOL CHEM, V267, P12188

[2] THE 3-DIMENSIONAL STRUCTURE OF AN ARACHIDONIC-ACID 15-LIPOXYGENASE [J].