CHARACTERIZATION OF CHEMOAUTOTROPHIC BACTERIAL SYMBIONTS IN A GUTLESS MARINE WORM (OLIGOCHAETA, ANNELIDA) BY PHYLOGENETIC 16S RIBOSOMAL-RNA SEQUENCE-ANALYSIS AND IN-SITU HYBRIDIZATION

被引:62
作者
DUBILIER, N
GIERE, O
DISTEL, DL
CAVANAUGH, CM
机构
[1] HARVARD UNIV, DEPT ORGANISM & EVOLUTIONARY BIOL, CAMBRIDGE, MA 02138 USA
[2] UNIV HAMBURG, INST ZOOL, D-20146 HAMBURG, GERMANY
[3] UNIV HAMBURG, ZOOL MUSEUM, D-20146 HAMBURG, GERMANY
[4] UNIV MAINE, DEPT BIOCHEM MICROBIOL & MOLEC BIOL, ORONO, ME 04469 USA
关键词
D O I
10.1128/AEM.61.6.2346-2350.1995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The phylogenetic relationships of chemoautotrophic endosymbionts in the gutless marine oligochaete Inanidrilus leukodermatus to chemoautotrophic ecto- and endosymbionts from other host phyla and to free-living bacteria were determined by comparative 16S rRNA sequence analysis. Fluorescent in situ hybridizations confirmed that the 16S rRNA sequence obtained from these worms originated from the symbionts. The symbiont sequence is unique to I. leukodermatus. In phylogenetic trees inferred by both distance and parsimony methods, the oligochaete symbiont is peripherally associated with one of two clusters of chemoautotrophic symbionts that belong to the gamma subdivision of the Proteobacteria. The endosymbionts of this oligochaete form a monophyletic group with chemoautotrophic ectosymbionts of a marine nematode. The oligochaete and nematode symbionts are very closely related, although their hosts belong to separate, unrelated animal phyla. Thus, cospeciation between the nematode and oligochaete hosts and their symbionts could not have occurred. Instead, the similar geographic locations and habitats of the hosts may have influenced the establishment of these symbioses.
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页码:2346 / 2350
页数:5
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