CLONING AND SEQUENCING THE CYTOSINE METHYLTRANSFERASE GENE M-CVIJL FROM CHLORELLA VIRUS IL-3A

被引：30

作者：

SHIELDS, SL ^{[1
]}

BURBANK, DE ^{[1
]}

GRABHERR, R ^{[1
]}

VANETTEN, JL ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT PLANT PATHOL,LINCOLN,NE 68588

来源：

VIROLOGY | 1990年 / 176卷 / 01期

关键词：

D O I：

10.1016/0042-6822(90)90225-G

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Chlorella virus IL-3A gene encoding the DNA methyltransferase M.CviJI, which methylates the internal cytosine in (G/A)GC(T/C/G) sequences, was cloned and expressed in Escherichia coli. The region containing the M.CviJI gene was sequenced and a single open reading frame of 1101 by was identified that could code for a polypeptide of 367 amino acids with a predicted molecular weight of 41,864. M.CviJI contained regions of amino acids which were similar to bacterial cytosine methyltransferases. Eighteen other Chlorella viruses, of 36 tested, contained DNA sequences which hybridized to the M.CviJI gene; DNA from some, but not all, of these 18 viruses also contained 5-methylcytosine in (G/A)GC(T/C/G) sequences. © 1990.

引用

页码：16 / 24

页数：9

共 27 条

[1] CLONING AND SEQUENCING OF A CDNA-ENCODING DNA METHYLTRANSFERASE OF MOUSE CELLS - THE CARBOXYL-TERMINAL DOMAIN OF THE MAMMALIAN ENZYMES IS RELATED TO BACTERIAL RESTRICTION METHYLTRANSFERASES [J].