DIVERSE EFFECTS OF POLYBASIC AMINO-ACIDS, HEPARIN AND IONIC-STRENGTH ON THE PHOSPHORYLATION OF VARIOUS SUBSTRATES BY CYTOSOLIC PROTEIN TYROSINE KINASE FROM PORCINE SPLEEN

被引：4

作者：

ASAHI, M ^{[1
]}

TANIGUCHI, T ^{[1
]}

SAKAI, K ^{[1
]}

NAKAMURA, S ^{[1
]}

YAMAMURA, H ^{[1
]}

机构：

[1] FUKUI MED SCH,DEPT BIOCHEM,FUKUI 91011,JAPAN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1990年 / 22卷 / 06期

关键词：

D O I：

10.1016/0020-711X(90)90041-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Effects of poly-basic amino acids, heparin and ionic strength on the activity of cytosolic protein-tyrosine kinase from porcine spleen (CPTK-40) have been studied. 2. 2. Both polylysine and polyarginine stimulated the phosphorylation of [Val5]angiotensin II and Eii|G1 (synthetic peptide of EDAEYAARRRG), but could neither stimulate nor inhibit the phosphorylation of random copolymers; poly(EY)4:1 and poly(EAY)6:3:1. 3. 3. Heparin stimulated the phosphorylation of poly(EY)4:1 by 2.5-fold, however, it inhibited those of E11,G1, poly(EAY)6:3:1, casein and H2B histone. 4. 4. Elevation of ionic strength of either NaCl, KC1 or (NH4)2SO4 stimulated the phosphorylation of poly(EY)4:1 >5-fold, but inhibited those of casein, tubulin. H2B histone, EiiGi and poly(EAY)6:3:1. 5. 5. These effectors did not change the Km for substrates but increased the Vmax. 6. 6. These results suggest that the effects of poly-basic amino acids, heparin and ionic strength on the activity of CPTK-40 are mainly on the substrates employed rather than on the enzyme itself. © 1990.

引用

页码：635 / 640

页数：6

共 32 条

[1] INTERACTIONS OF THE HUMAN RED-CELL MEMBRANE TYROSINE KINASE WITH HEPARIN [J].