PREPARATION OF HUMAN MANGANESE SUPEROXIDE-DISMUTASE BY TRI-PHASE PARTITIONING AND PRELIMINARY CRYSTALLOGRAPHIC DATA

被引：12

作者：

DEUTSCH, HF

HOSHI, S

MATSUDA, Y

SUZUKI, K

KAWANO, K

KITAGAWA, Y

KATSUBE, Y

TANIGUCHI, N

机构：

[1] OSAKA UNIV,SCH MED,DEPT BIOCHEM,2-2 YAMADAOKA,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,INST PROT RES,SUITA,OSAKA 565,JAPAN

[3] OSAKA ROSAI HOSP,SAKAI 591,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 01期

关键词：

MN SUPEROXIDE DISMUTASE; TRI-PHASE PARTITIONING; CRYSTALLIZATION; PRELIMINARY X-RAY DATA; HUMAN LIVER;

D O I：

10.1016/0022-2836(91)90860-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human liver manganese superoxide dismutase has been purified by a short procedure that includes a tri-phase partitioning step to provide materials that can be crystallized from ammonium sulfate. X-ray diffraction studies at 3 Å resolution show that the crystals belong to the hexagonal space group P6122 or P6522, with cell dimensions a = b = 81.1 A ̊, c = 242.2 A ̊. Manganese superoxide dismutase levels as determined by enzymatic assay as well as by enzyme-linked immunosorbent assay indicated that considerable variations occur in different livers but the total superoxide dismutase activity (Mn superoxide dismutase plus Cu,Zn superoxide dismutase) seems to be kept at constant values. © 1991.

引用

页码：103 / 108

页数：6

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