SUBSTRATE-SPECIFICITY AND POSITIONAL PREFERENCE OF A LIPOPROTEIN-LIPASE

The substrate specificity and positional preference of a membrane-bound lipoprotein lipase with and without reconstitution in liposomes were studied. The enzyme showed the same preference for the acyl groups in the 1- and 3-positions of triglycerides, while its activity toward the 2-position was one-third of that toward the 1- or 3-positions. The substrate specificity of the enzyme toward fatty acids of different chain length and degree of unsaturation was in the order of C:14>C:16>C:18:1greater-than-or-equal-toC:18. The enzyme with and without reconstitution showed the same positional preference and substrate specificity.