THYMIC SELECTION OF CYTOTOXIC T-CELLS INDEPENDENT OF CD8-ALPHA-LCK ASSOCIATION

被引：67

作者：

CHAN, IT

LIMMER, A

LOUIE, MC

BULLOCK, ED

FUNGLEUNG, WP

MAK, TW

LOH, DY

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MED GENET & MOLEC MICROBIOL,HOWARD HUGHES MED INST,ST LOUIS,MO 63110

[2] ONTARIO CANC INST,TORONTO M4X 1K9,ONTARIO,CANADA

[3] UNIV TORONTO,DEPT MED BIOPHYS,TORONTO M4X 1K9,ONTARIO,CANADA

[4] UNIV TORONTO,DEPT IMMUNOL,TORONTO M4X 1K9,ONTARIO,CANADA

来源：

SCIENCE | 1993年 / 261卷 / 5128期

关键词：

D O I：

10.1126/science.8372352

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The CD8alpha cytoplasmic domain associates with p56lck, a nonreceptor protein-tyrosine kinase. The biological relevance of CD8alpha-Lck association in T cell development was tested with transgenic mice generated to express a CD8alpha molecule with two amino acid substitutions in its cytoplasmic domain, which abolishes the association of CD8alpha with Lck. The CD8alpha mutant was analyzed in a CD8-/- background and in the context of the transgenic 2C T cell receptor. The development and function of CD8+ T cells in these mice were apparently normal. Thus, CD8alpha-Lck association is not necessary for positive selection, negative selection, or CD8-dependent cytotoxic function.

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页码：1581 / 1584

页数：4

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