SEPARATION OF LARGE DNA FRAGMENTS BY CAPILLARY ELECTROPHORESIS UNDER PULSED-FIELD CONDITIONS

被引：60

作者：

SUDOR, J ^{[1
]}

NOVOTNY, MV ^{[1
]}

机构：

[1] INDIANA UNIV, DEPT CHEM, BLOOMINGTON, IN 47405 USA

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 15期

关键词：

D O I：

10.1021/ac00087a007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary zone electrophoresis (CZE), using an entangled polyacrylamide solution, was applied to large DNA samples under pulsed-field conditions. Highly efficient separations were achieved under biased sinusoidal field and field-inversion pulsing regimes. The separations that were obtained with 8.3-48.5 kb lambda DNA standards and 48.5 kb-1 Mb lambda DNA concatamers (modified with ethidium bromide) clearly demonstrate a dramatic improvement in the separation time (roughly, 10-50 times) over the conventionally used slab-gel techniques. Moreover, the CZE method appears much more sensitive and amenable to component quantification and method automation.

引用

收藏

页码：2446 / 2450

页数：5

相关论文

共 35 条

[1] INFLUENCE OF SOLID FRICTION ON POLYMER RELAXATION IN GEL-ELECTROPHORESIS [J].

BURLATSKY, S ;

DEUTCH, J .

SCIENCE, 1993, 260 (5115) :1782-1784

[2] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD [J].

CARLE, GF ;

FRANK, M ;

OLSON, MV .

SCIENCE, 1986, 232 (4746) :65-68

[3] ELECTROPHORETIC SEPARATIONS OF PROTEINS IN CAPILLARIES WITH HYDROLYTICALLY STABLE SURFACE-STRUCTURES [J].

COBB, KA ;

DOLNIK, V ;

NOVOTNY, M .

ANALYTICAL CHEMISTRY, 1990, 62 (22) :2478-2483

[4] REPTATION OF A POLYMER CHAIN IN PRESENCE OF FIXED OBSTACLES [J].

DEGENNES, PG .

JOURNAL OF CHEMICAL PHYSICS, 1971, 55 (02) :572-+

[5] IMPROVED SEPARATION OF NUCLEIC-ACIDS WITH ANALYTE VELOCITY MODULATION CAPILLARY ELECTROPHORESIS [J].

DEMANA, T ;

LANAN, M ;

MORRIS, MD .

ANALYTICAL CHEMISTRY, 1991, 63 (23) :2795-2797

[6] EXPLANATION OF ANOMALOUS MOBILITY AND BIREFRINGENCE MEASUREMENTS FOUND IN PULSED FIELD ELECTROPHORESIS [J].

DEUTSCH, JM .

JOURNAL OF CHEMICAL PHYSICS, 1989, 90 (12) :7436-7441

[7]

DOI M, 1978, J CHEM SOC FARAD T 2, V74, P1789, DOI 10.1039/f29787401789

[8] MOTION OF MEGABASE DEOXYRIBONUCLEIC-ACID DURING FIELD-INVERSION GEL-ELECTROPHORESIS - INVESTIGATION BY NONLOCAL MONTE-CARLO [J].

DUKE, TAJ ;

VIOVY, JL .

JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (11) :8552-8563

[9] EXPERIMENTAL AND THEORETICAL-STUDIES OF DNA SEPARATIONS BY CAPILLARY ELECTROPHORESIS IN ENTANGLED POLYMER-SOLUTIONS [J].

GROSSMAN, PD ;

SOANE, DS .

BIOPOLYMERS, 1991, 31 (10) :1221-1228

[10] CAPILLARY ZONE ELECTROPHORESIS OF LARGE DNA [J].

GUSZCZYNSKI, T ;

PULYAEVA, H ;

TIETZ, D ;

GARNER, MM ;

CHRAMBACH, A .

ELECTROPHORESIS, 1993, 14 (5-6) :523-530

← 1 2 3 4 →