Co-selection of cognate antibody-antigen pairs by selectively-infective phages

被引：59

作者：

Krebber, C ^{[1
]}

Spada, S ^{[1
]}

Desplancq, D ^{[1
]}

Pluckthun, A ^{[1
]}

机构：

[1] UNIV ZURICH,INST BIOCHEM,CH-8057 ZURICH,SWITZERLAND

来源：

FEBS LETTERS | 1995年 / 377卷 / 02期

关键词：

selectively-infective phage; phage display; antibody engineering; filamentous phage; single-chain Fv fragment;

D O I：

10.1016/0014-5793(95)01348-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected, The phage genome encodes a fusion of single-chain antibody to the C-terminal domain of gIIIp, rendering the phage non-infective, The antigen fused to the N-terminal domains of gIIIp is encoded in the same phage genome, Antigen and antibody fusion interact with each other in the periplasm of the phage-producing cell, restoring infectivity, This system has a very low background and will allow simultaneous randomisation of antibody and antigen.

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收藏

页码：227 / 231

页数：5

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