DNA-FINGERPRINTING IN SILKWORM BOMBYX-MORI USING BANDED KRAIT MINOR SATELLITE DNA-DERIVED PROBE

被引：17

作者：

NAGARAJU, J ^{[1
]}

SHARMA, A ^{[1
]}

SETHURAMAN, BN ^{[1
]}

RAO, GV ^{[1
]}

SINGH, L ^{[1
]}

机构：

[1] CTR CELLULAR & MOLEC BIOL, HYDERABAD 500007, ANDHRA PRADESH, INDIA

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 09期

关键词：

D O I：

10.1002/elps.11501601271

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The genomic DNA from thirteen different ecotypes and inbred lines of silkworm, Bombyx mori, were analyzed by digesting with BstNI and HinfI restriction enzymes followed by hybridization with banded krait minor satellite DNA (Bkm)-2(8) minisatellite probe. The DNA fingerprinting revealed 9-31 discrete intense bands, some of which were ecotype/inbred line-specific. Individual specific DNA fingerprints in two representative genotypes and their F-1 hybrid offspring were also obtained. Individuals of a given parental line showed very similar profiles and the hybrid offspring showed the combined profile of both parents. The presence of bands specific to diapausing and nondiapausing strains and to particular genotypes indicate their potential use for marker-assisted breeding and varietal identification.

引用

页码：1639 / 1642

页数：4

共 19 条

[1] GENERIC AFFINITIES AMONG CROCODILIANS AS REVEALED BY DNA-FINGERPRINTING WITH A BKM-DERIVED PROBE [J].

AGGARWAL, RK ;

MAJUMDAR, KC ;

LANG, JW ;

SINGH, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (22) :10601-10605

[2] DNA FINGERPRINTING IN REPTILES - BKM HYBRIDIZATION PATTERNS IN CROCODILIA AND CHELONIA [J].

DEMAS, S ;

WACHTEL, S .

GENOME, 1991, 34 (03) :472-476

[3] SEX-SPECIFIC DNA IN REPTILES WITH TEMPERATURE SEX DETERMINATION [J].

DEMAS, S ;

DURONSLET, M ;

WACHTEL, S ;

CAILLOUET, C ;

NAKAMURA, D .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1990, 253 (03) :319-324

[4]

GAMO T, 1993, JPN AGR RES Q, V16, P264

[5]

HIROBE T, 1968, P C GENETICS TOKYO, P25

[6]

HU NT, 1982, GENE, V17, P271

[7] INDIVIDUALIZATION AND ESTIMATION OF RELATEDNESS IN CROCODILIANS BY DNA FINGERPRINTING WITH A BKM-DERIVED PROBE [J].

LANG, JW ;

AGGARWAL, RK ;

MAJUMDAR, KC ;

SINGH, L .

MOLECULAR AND GENERAL GENETICS, 1993, 238 (1-2) :49-58

[8]

MUKHERJEE NG, 1919, HDB SERICULTURE

[9] GENOME FINGERPRINTING OF THE SILKWORM, BOMBYX-MORI, USING RANDOM ARBITRARY PRIMERS [J].

NAGARAJA, GM ;

NAGARAJU, J .

ELECTROPHORESIS, 1995, 16 (09) :1633-1638

[10]

NAGARAJU J, 1995, J SERIC SCI JPN, V64, P1

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