GTP-DEPENDENT POLYMERIZATION OF ESCHERICHIA-COLI FTSZ PROTEIN TO FORM TUBULES

被引：229

作者：

BRAMHILL, D

THOMPSON, CM

机构：

[1] Department of Enzymology, Building 80Y-325, Merck Research Laboratories, Rahway, NJ 07065-0900

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 13期

关键词：

CYTOKINESIS; SEPTUM SYNTHESIS; CYTOSKELETON; TUBULIN-LIKE; MICROTUBULE;

D O I：

10.1073/pnas.91.13.5813

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The FtsZ protein is a GTPase that is essential for cell division in Escherichia coli. During cytokinesis, FtsZ localizes to a ring at the leading edge of septum synthesis. We report the GTP-dependent polymerization of purified FtsZ measured by sedimentation and light scattering. Electron microscopy of polymerized FtsZ revealed structures including tubules 14-20 nm in diameter with longitudinal arrays of protofilaments. FtsZ depolymerized upon removal of GTP and repolymerized after subsequent GTP addition. Mutant FtsZ84 protein polymerized inefficiently, suggesting that polymerization is important for the cellular role of FtsZ in division. The possibility that tubules of FtsZ protein form a cytoskeleton involved in septum synthesis is consistent with our data.

引用

页码：5813 / 5817

页数：5

共 46 条

[1]

AMOS LA, 1974, J CELL SCI, V14, P523

[2] ALKALINE-PHOSPHATASE FUSIONS IN THE STUDY OF CELL-DIVISION GENES [J].