ANDROGEN RECEPTORS IN HEPATOCELLULAR-CARCINOMA AND SURROUNDING LIVER - RELATIONSHIP WITH TUMOR SIZE AND RECURRENCE RATE AFTER SURGICAL RESECTION

Background/Aims: This study aimed to evaluate the parameters associated with the presence of androgen receptors in hepatocellular carcinoma and surrounding non-tumoral liver, Furthermore, me have assessed whether androgen receptor positivity influences disease recurrence after surgical resection. Methods: Androgen receptor concentration was calculated by receptor binding assay in tumoral and nontumoral liver in 43 patients (40 of them with cirrhosis) with hepatocellular carcinoma who underwent surgical resection. Results: Androgen receptors were found in 28 of the tumoral and in 30 of the non-tumoral samples, at concentrations ranging between 5 and 211 fmol/mg protein. The presence of androgen receptors within the tumor was significantly related to a smaller tumor size. Thereby, 22 of the 29 nodules less than or equal to 3 cm contained androgen receptors, while this occurred in only six of the 14 tumors larger than 3 cm (p<0.05). Tn contrast, the only parameter associated with the presence of androgen receptors in the non-tumoral liver was a lower gamma-glutamyltranspeptidase concentration. Disease recurrence after surgical resection was not only related to some tumor characteristics (increased alfa-fetoprotein concentration, presence of satellites, differentiation degree), but also to the presence of androgen receptors in the surrounding liver. Thus, the probability of recurrence after 1- and a-year follow up in patients with androgen-positive livers was 33% and 50%, respectively, while it was 0% and 20% in those with androgen-negative livers (p<0.05), In contrast, the presence of androgen receptors within the tumor was not associated with a higher recurrence rate. Conclusions: These results show that only two thirds of hepatocellular carcinomas contained androgen receptors and that this feature was more frequent in small tumors. In addition, our data indicate that the presence of androgen receptors within the tumor does not imply a different outcome after surgical resection. In contrast, the presence of these receptors in the surrounding non-tumoral liver may be considered a risk factor for a higher incidence of disease recurrence.