AN ELECTRONIC INTERFACE FOR MICROCOMPUTER-BASED FLOW-CYTOMETRY - DESIGN, OPERATION AND PERFORMANCE-CHARACTERISTICS

被引：2

作者：

DVORAK, JA

MUDD, CP

DVORAK, VK

SCHUETTE, WH

机构：

[1] DAVID TAYLOR RES CTR,DEPT ELECTROMAGNET SIGNATURES,BETHESDA,MD 20084

[2] NIH,DIV RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892

[3] NIAID,PARASIT DIS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF MICROCOMPUTER APPLICATIONS | 1991年 / 14卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0745-7138(91)90019-N

中图分类号：

TP3 [计算技术、计算机技术];

学科分类号：

0812 ;

摘要：

An inexpensive electronic interface for the acquisition of asynchronous flow cytometry data using a commercially available microcomputer and A/D converter is described. In the design of this interface, the major objectives were to minimize the amount of custom hardware necessary between the computer and flow cytometer and to maximize usable sensitivity and resolution by implementing a gated integrator and trigger hold-off approach to acquire the asynchronous data pulses from the cytometer. The major error sources which can occur during data acquisition are identified and their effects on system performance are shown. A simple protocol is described to minimize the effects of these errors on the acquired data. The influence of computer-processor clock speed on data acquisition rates is also documented. © 1991.

引用

页码：327 / 341

页数：15