CRYSTALLIZATION OF THE EXO(1,3)-BETA-GLUCANASE FROM CANDIDA-ALBICANS

被引：7

作者：

CUTFIELD, S ^{[1
]}

BROOKE, G ^{[1
]}

SULLIVAN, P ^{[1
]}

CUTFIELD, J ^{[1
]}

机构：

[1] UNIV OTAGO,DEPT BIOCHEM,POB 56,DUNEDIN,NEW ZEALAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 225卷 / 01期

关键词：

EXOGLUCANASE; CANDIDA-ALBICANS; CRYSTALLIZATION; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1016/0022-2836(92)91037-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An exoglucanase, with specificity for β(1,3) linkages, from the cell wall of Candida albicans has been crystallized by the hanging drop method in the presence of polyethylene glycol 8000. The crystals, which diffract to better than 1.9 Å resolution, belong to the orthorhombic space group P212121 with cell constants a = 60.2 A ̊, b = 65.2 A ̊, c = 96.5 A ̊ and with one molecule in the asymmetric unit. © 1992.

引用

页码：217 / 218

页数：2

共 11 条

[1] THE GLU RESIDUE IN THE CONSERVED ASN-GLU-PRO SEQUENCE OF 2 HIGHLY DIVERGENT ENDO-BETA-1,4-GLUCANASES IS ESSENTIAL FOR ENZYMATIC-ACTIVITY
BAIRD, SD
HEFFORD, MA
JOHNSON, DA
SUNG, WL
YAGUCHI, M
SELIGY, VL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 169 (03) : 1035 - 1039
[2] 3 DIMENSIONAL STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE AT 2.9 A RESOLUTION - ROLE OF CALCIUM IN STRUCTURE AND ACTIVITY
BUISSON, G
DUEE, E
HASER, R
PAYAN, F
[J]. EMBO JOURNAL, 1987, 6 (13) : 3909 - 3916
[3] CHAMBERS RS, 1991, CANDIDA ALBICANS XOG
[4] DEALDANA CRV, 1991, J BACTERIOL, V171, P6259
[5] STRUCTURE AND POSSIBLE CATALYTIC RESIDUES OF TAKA-AMYLASE A
MATSUURA, Y
KUSUNOKI, M
HARADA, W
KAKUDO, M
[J]. JOURNAL OF BIOCHEMISTRY, 1984, 95 (03) : 697 - 702
[6] SOLVENT CONTENT OF PROTEIN CRYSTALS
MATTHEWS, BW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) : 491 - +
[7] NOMBELA C, 1988, MICROBIOL SCI, V5, P328
[8] 3-DIMENSIONAL STRUCTURE OF CELLOBIOHYDROLASE-II FROM TRICHODERMA-REESEI
ROUVINEN, J
BERGFORS, T
TEERI, T
KNOWLES, JKC
JONES, TA
[J]. SCIENCE, 1990, 249 (4967) : 380 - 386
[9] X-RAY-DIFFRACTION DATA-COLLECTION SYSTEM FOR MODERN PROTEIN CRYSTALLOGRAPHY WITH A WEISSENBERG CAMERA AND AN IMAGING PLATE USING SYNCHROTRON RADIATION
SAKABE, N
[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 1991, 303 (03) : 448 - 463
[10] SINNOTT ML, 1987, ENZYME MECHANISMS, P259

← 1 2 →