CHIRAL SEPARATIONS USING DEXTRAN AND BOVINE SERUM-ALBUMIN AS RUN BUFFER ADDITIVES IN AFFINITY CAPILLARY ELECTROPHORESIS

被引：71

作者：

SUN, P ^{[1
]}

WU, N ^{[1
]}

BARKER, G ^{[1
]}

HARTWICK, RA ^{[1
]}

机构：

[1] SUNY BINGHAMTON,DEPT CHEM,BINGHAMTON,NY 13902

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 648卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80431-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method utilizing dextran as a run buffer additive in addition to bovine serum albumin (BSA) for chiral separation by means of affinity capillary electrophoresis (ACE) has been developed. By adding different amounts of dextran to the run buffer, the net velocity of BSA can be adjusted to a desired rate. Enantiomers of some drugs [ibuprofen (IB) and leucovorin (LV)] and amino acids (dansyl-DL-leucine and dansyl-DL-norvaline) were separated on a capillary with 20 cm effective length by this method. The effect of dextran concentration on the retention of BSA and resolution of sample enantiomers was studied. Enantiomers of mandelic acid (MA), which have very weak affinity for BSA, were also resolved. Qualitative information pertaining to the binding interaction of the samples with BSA can be obtained by this method.

引用

页码：475 / 480

页数：6

共 25 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].