SIGNALING BY E-SELECTIN AND ICAM-1 INDUCES ENDOTHELIAL TISSUE FACTOR PRODUCTION VIA AUTOCRINE SECRETION OF PLATELET-ACTIVATING-FACTOR AND TUMOR-NECROSIS-FACTOR-ALPHA

被引:31
作者
SCHMID, E
MULLER, TH
BUDZINSKI, RM
BINDER, K
PFIZENMAIER, K
机构
[1] DR KARL THOMAE GMBH,DEPT BIOCHEM RES,D-88397 BIBERACH,GERMANY
[2] DR KARL THOMAE GMBH,DEPT PHARMACOL RES,D-88397 BIBERACH,GERMANY
关键词
D O I
10.1089/jir.1995.15.819
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Based on previous studies showing adhesion molecule-dependent induction of tissue factor upon endothelium-lymphocyte interactions, we investigated whether E-selectin and ICAM-1 are linked to signaling pathways leading to tissue factor gene expression. Cellular interaction was mimicked by antibody cross-linking of E-selectin and ICAM-1 on the surface of human umbilical vein endothelial cells (HUVECs), resulting in induction of tissue factor mRNA and protein expression. Tissue factor production could be Independently abolished by antibodies against TNF-alpha and by WEB 2086, a platelet-activating factor (PAF) receptor antagonist, Because WEB 2086 prevented the production and/or secretion of TNF-alpha by HUVECs, these results provide evidence for E-selectin- and ICAM-1-linked signal pathways leading to tissue factor synthesis in endothelial cells via an autocrine feedback loop involving PAF and TNF-alpha secretion.
引用
收藏
页码:819 / 825
页数:7
相关论文
共 41 条
[1]  
KIRCHHOFER D., SAKARIASSEN K., CLOZEL M., TSCHOPP T., HADVARY R., NEMERSON Y., BAUMGARTNER H., Relationship between tissue factor expression and deposition of fibrin, platelets and leukocytes on cultured endothelial cells under venous blood flow conditions, Blood, 81, (1993)
[2]  
WARR T., RAO L., RAPAPORT S., Disseminated intravascular coagulation in rabbits induced by administration of endotoxin or tissue factor: effect of antitissue factor antibodies and measurement of plasma extrinsic pathway inhibitor activity, Blood, 75, (1990)
[3]  
TAYLOR F.B.J., CHANG A., RUF W., MORRISSEY J.H., HINSHAW L., CATLETT R., BLICK K., EDGINGTON T.S., Letal E. coli septic shock is prevented by blocking tissue factor with monoclonal antibody, Circ. Shock, 33, (1991)
[4]  
BAUER K.A., KASS B.L., CATE H.T., HAWIGER J.J., ROSENBERG R.D., Factor IX is activated in vivo by the tissue factor mechanism, Blood, 76, (1990)
[5]  
NEMERSON Y., The reaction between bovine brain tissue factor and factors VII and X, Biochemistry, 5, (1966)
[6]  
KOMIYAMA Y., PEDERSEN A.H., KISIEL W., Proteolytic activation of human factors IX and X by recombinant human factor Vila: effects of calcium, phospholipids, and tissue factor, Biochemistry, 29, (1990)
[7]  
GALDAL K.S., Thromboplastin synthesis in endothelial cells, Haemostasis, 14, (1984)
[8]  
LYBERG T., GALDAL K.S., EVENSEN S.A., PRYDZ H., Thromboplastin (factor III) activity in human monocytes induced by immune complexes, Br. J. Haematol., 53, (1982)
[9]  
CARLSEN E., GAUDERNACK G., FILION-MYKLEBUST C., PETTERSEN K.S., PRYDZ H., Allogeneic induction of thromboplastin synthesis in monocytes and endothelial cells, Biphasic effect of cyclosporin A. Clin. Exp. Inmiunol., 76, (1989)
[10]  
BINDER K., SCHMID E., MULLER T.H., DIC: Pathogenesis, Diagnosis and Therapy of Disseminated Intravascular Fibrin Formation, (1993)