MICROTUBULE ORGANIZATION IN CULTURED SOYBEAN AND BLACK SPRUCE CELLS - INTERPHASE MITOSIS TRANSITION AND SPINDLE MORPHOLOGY

被引:13
作者
WANG, H
CUTLER, AJ
FOWKE, LC
机构
[1] UNIV SASKATCHEWAN,DEPT BIOL,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA
[2] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA
关键词
PICEA-MARIANA; CELL CYCLE; IMMUNOFLUORESCENCE MICROSCOPY; MICROTUBULE ORGANIZATION; SPINDLE; GLYCINE-MAX (L) MERR;
D O I
10.1007/BF01403900
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Microtubule (MT) distribution during the cell cycle, especially spindle organization, has been investigated using immunofluorescence light microscopy in cultured cells of two higher plant species, soybean (angiosperm) and black spruce (gymnosperm). In soybean, the prophase and metaphase spindles were different in morphology and structure. The prophase spindle covering the nucleus was barrel-shaped and MTs extended between poles. The metaphase spindle consisted mainly of short MT bundles on either side of the chromosome mass. During prometaphase, the polarity and shape of the prophase spindle disappeared, suggesting that the metaphase spindle is newly formed in prometaphase and not derived from the prophase spindle. A striking feature of MT organization in black spruce was sharply defined poles during prometaphase and anaphase. They were located close to the cell edge, suggesting that a structure in the cytoplasm or associated with the plasma membrane is responsible for their formation. In black spruce the metaphase spindle was long with pointed poles and MT "fir tree" structures. In contrast, the metaphase spindle of soybean was short with very broad poles and lacked MT "fir trees". These results suggest that MT fir tree structure may not be necessary for a functional spindle.
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页码:46 / 54
页数:9
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