DETECTION AND SOME PROPERTIES OF THE SIALYLTRANSFERASE IMPLICATED IN THE SIALYLATION OF LIPOPOLYSACCHARIDE OF NEISSERIA-GONORRHOEAE

被引：38

作者：

MANDRELL, RE

SMITH, H

JARVIS, GA

GRIFFISS, JM

COLE, JA

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

[2] VET ADM MED CTR,CTR IMMUNOCHEM,SAN FRANCISCO,CA 94121

[3] UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143

[4] UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143

来源：

MICROBIAL PATHOGENESIS | 1993年 / 14卷 / 04期

关键词：

NEISSERIA-GONORRHOEAE; GONOCOCCI; SIALYLTRANSFERASE; LIPOOLIGOSACCHARIDE (LOS);

D O I：

10.1006/mpat.1993.1030

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A sensitive assay for sialyltransferase (STase) activity extracted from gonococci with 0.5% Triton X100 was developed. Enzyme activity was optimal in the pH range 5.8-8.0 and was strongly inhibited by CMP, CDP and CTP, but not by other nucleotides, 10 mM Mg2+, Zn2+, Ca2+ or Mn2+, or by 18 mM EDTA. More than 90% of the activity was lost after 30 s at 67°C. The apparent Vmax and apparent Km of the STase for cytidine 5´-monophospho-N-acetylneuraminic acid were 1.7 nmol of NANA incorporated/min/mg protein and 5.3 µM, respectively. © 1993 Academic Press.

引用

页码：307 / 313

页数：7

共 20 条

[1] MODIFICATION BY SIALIC-ACID OF NEISSERIA-GONORRHOEAE LIPOOLIGOSACCHARIDE EPITOPE EXPRESSION IN HUMAN URETHRAL EXUDATES - AN IMMUNOELECTRON MICROSCOPIC ANALYSIS [J].