PRECISE LARGE DELETIONS BY THE PCR-BASED OVERLAP EXTENSION METHOD

被引：70

作者：

SENANAYAKE, SD ^{[1
]}

BRIAN, DA ^{[1
]}

机构：

[1] UNIV TENNESSEE,DEPT MICROBIOL,KNOXVILLE,TN 37996

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 01期

关键词：

PCR; OVERLAP EXTENSION; PRECISE LARGE DELETIONS;

D O I：

10.1007/BF02907467

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors describe an efficient method for generating large deletions; (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (I). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded: DNA template.

引用

页码：13 / 15

页数：3