AUTOLIGATION OF OLIGONUCLEOTIDES VIA NUCLEOPHILIC-SUBSTITUTION REACTION

被引：5

作者：

GRYAZNOV, SM

机构：

[1] Lynx Therapeutics, Inc., Hayward, CA, 94545

来源：

NUCLEOSIDES & NUCLEOTIDES | 1995年 / 14卷 / 3-5期

关键词：

D O I：

10.1080/15257779508012524

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Fast and efficient template - driven autoligation reaction between oligonucleotides derivatized with bromoacetyl and thiol groups at their opposing termini is described. The product of reaction is capable of forming a stable duplex with a complementary DNA strand.

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页码：1019 / 1022

页数：4

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