DIFFERENTIAL EXPRESSION OF 2 BARLEY SNF1-RELATED PROTEIN-KINASE GENES

被引：43

作者：

HANNAPPEL, U ^{[1
]}

VICENTECARBAJOSA, J ^{[1
]}

BARKER, JHA ^{[1
]}

SHEWRY, PR ^{[1
]}

HALFORD, NG ^{[1
]}

机构：

[1] UNIV BRISTOL,LONG ASHTON RES STN,DEPT AGR SCI,INST ARABLE CROPS RES,BRISTOL BS18 9AF,AVON,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 27卷 / 06期

关键词：

GENE EXPRESSION; GENE FAMILY; HIGHER PLANTS; HORDEUM VULGARE; METABOLIC REGULATION;

D O I：

10.1007/BF00020898

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have amplified and cloned DNA sequences derived from a gene encoding a SNF1 (sucrose-nonfermenting 1)-related protein kinase which differs from that previously reported from barley. Northern blot and polymerase chain reaction (PCR) analysis of RNA populations, using specific probes and oligonucleotide primers, indicated that the two SNF1-related genes are differentially regulated. One is expressed in all tissues, whereas the other is expressed at high levels in the seed endosperm and aleurone, but at levels undetectable by northern blot analysis in other tissues. Comparisons with other plant SNF1-related protein kinase genes suggest that the form which is expressed at greatly enhanced levels in the seed is less similar to the other plant homologues which have been reported and may be unique to cereals.

引用

页码：1235 / 1240

页数：6

共 28 条

[1] COMPLEMENTATION OF SNF1, A MUTATION AFFECTING GLOBAL REGULATION OF CARBON METABOLISM IN YEAST, BY A PLANT PROTEIN-KINASE CDNA [J].