DEVELOPMENT OF A CONSENSUS LINKAGE RFLP MAP OF CULTIVATED SUNFLOWER (HELIANTHUS-ANNUUS L)

被引：125

作者：

GENTZBITTEL, L

VEAR, F

ZHANG, YX

BERVILLE, A

NICOLAS, P

机构：

[1] INRA,GENET & AMELIORAT PLANTES STN,F-63039 CLERMONT FERRAND,FRANCE

[2] ECOLE NATL SUPER AGRON MONTIPELLIER,INRA,SGAP,F-34060 MONTPELLIER 1,FRANCE

[3] UNIV CLERMONT FERRAND,INRA,ORG & VARIABIL GENOMES VEGETAUX,F-63038 CLERMONT FERRAND,FRANCE

[4] GIE CARTISOL,F-75008 PARIS,FRANCE

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 7-8期

关键词：

RFLP; HELIANTHUS ANNUUS; LINKAGE MAP; CONSENSUS MAP; CDNA;

D O I：

10.1007/BF00222925

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This paper provides the first description of a consensus map of the cultivated sunflower genome (Helianthus annuus L., n=17 chromosomes), based on RFLP. A total of 180 probe-enzyme combinations were mapped on at least one of five segregating progenies (three F-2 and two BC1 populations), revealing 237 loci that did not show any distortion of segregation. The consensus linkage map obtained with these loci covers 1150 cM and consists of 16 linkage groups of more than 20 cM, 7 groups of less than 20 cM and 18 unlinked loci. The mean distance between loci is 7 cM, but in some regions intervals of 20 cM remain. Genotypic and gametic segregation distortions affect about 7% of loci. It was found that 25% of the probes mapped using several different restriction enzymes or that on different progenies they revealed 2 or more loci.

引用

页码：1079 / 1086

页数：8

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