RAPID ANALYSIS OF MOUSE - HAMSTER HYBRID CELL-LINES BY INSITU HYBRIDIZATION

被引：25

作者：

BOYLE, AL ^{[1
]}

LICHTER, P ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510

来源：

GENOMICS | 1990年 / 7卷 / 01期

关键词：

D O I：

10.1016/0888-7543(90)90529-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In situ htbridization techniques for analyzing the murine DNA complement of mouse-hamster hybrid cells are described. Total genomic mouse DNA is labeled with biotin and hybridized without suppression to metaphase spreads from a mouse-hamster hybrid line containing the mouse fusion chromosome X12. Detection via fluorochrome-conjugated avidin reveals mouse chromosomal DNA with high sensitivity and permits the identification of both normal and aberrant murine chromosomes. Conversely, biotinylated total genomic DNA from a hybrid line can be used as a probe on normal mouse metaphase spreads if suppression techniques are employed, facilitating the analysis of mouse chromosomes present in the hybrid line. © 1990.

引用

页码：127 / 130

页数：4

共 17 条

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