ENZYME SENSOR-FIA-SYSTEM FOR ONLINE MONITORING OF GLUCOSE, LACTATE AND GLUTAMINE IN ANIMAL-CELL CULTURES

被引：42

作者：

RENNEBERG, R

TROTTKRIEGESKORTE, G

LIETZ, M

JAGER, V

PAWLOWA, M

KAISER, G

WOLLENBERGER, U

SCHUBERT, F

WAGNER, R

SCHMID, RD

SCHELLER, FW

机构：

[1] GESELL BIOTECHNOL FORSCH GMBH,BEREICH ENZYMTECHNOL,MASCHERODER WEG 1,W-3300 BRAUNSCHWEIG,GERMANY

[2] ZENT INST MOLEK BIOL,BERLIN,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1991年 / 21卷 / 1-2期

关键词：

FIA; ENZYME SENSORS FOR GLUCOSE; LACTATE; GLUTAMINE; ONLINE BIOREACTOR CONTROL; HYBRIDOMA CELL CULTURE; RECOMBINANT BHK CELLS;

D O I：

10.1016/0168-1656(91)90269-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enzyme sensors for glucose, lactate and glutamine were connected via flow-injection analysis (FIA) devices to two different bioprocesses. They were used for on-line process control of perfused bioreactor systems containing mammalian cell lines producing a monoclonal antibody and recombinant interleukin-2. The biosensor system gives direct access to important process data which can be used as control parameters for long term cell cultivation systems.

引用

页码：173 / 186

页数：14

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