EFFECTS OF STRESS AND ADRENALECTOMY ON TYROSINE-HYDROXYLASE MESSENGER-RNA LEVELS IN THE LOCUS-CERULEUS BY INSITU HYBRIDIZATION

被引:118
作者
SMITH, MA [1 ]
BRADY, LS [1 ]
GLOWA, J [1 ]
GOLD, PW [1 ]
HERKENHAM, M [1 ]
机构
[1] NIMH, FUNCT NEUROANAT SECT, BLDG 36, RM 2D15, BETHESDA, MD 20892 USA
关键词
TYROSINE HYDROXYLASE; LOCUS CERULEUS; INSITU HYBRIDIZATION; MESSENGER RIBONUCLEIC ACID; FOOTSHOCK STRESS; RESTRAINT STRESS; ADRENALECTOMY;
D O I
10.1016/0006-8993(91)90881-U
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The locus ceruleus-norepinephrine system is one of the principal effectors of the stress response. Acute stress induces norepinephrine synthesis and release, and noradrenergic cells compensate by increasing the activity of tyrosine hydroxylase, the rate-limiting enzyme in catecholamine synthesis. Here we use in situ hybridization histochemistry to show the effects of acute and chronic intermittent stress on the expression of tyrosine hydroxylase mRNA in the rat locus cereleus. Restraint stress increased tyrosine hydroxylase mRNA in the locus ceruleus but not in dopaminergic nuclei such as the substantia nigra or ventral tegmental area. One hour of footshock or restraint caused a rapid increase in locus ceruleus tyrosine hydroxylase mRNA which returned to basal levels within 24 h. Chronic intermittent stress (1 hour of restraint or footshock per day for 14 days) produced no change in tyrosine hydroxylase mRNA. Neither adrenalectomy nor dexamethasone replacement significantly affected mRNA expression. These findings indicate that acute stress can increase the expression of tyrosine hydroxylase mRNA in the locus ceruleus but that adaptation occurs to repeated stress, and that the expression of tyrosine hydroxylase mRNA in the locus ceruleus is independent of direct glucocorticoid modulation.
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页码:26 / 32
页数:7
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