A RAPID METHOD FOR THE QUANTIFICATION OF STEROIDAL GLYCOALKALOIDS BY REVERSED PHASE HPLC

被引：25

作者：

JONKER, HH ^{[1
]}

KOOPS, AJ ^{[1
]}

HOOGENDOORN, JC ^{[1
]}

机构：

[1] DLO,CTR PLANT BREEDING & REPROD RES,POB 16,6700 AA WAGENINGEN,NETHERLANDS

来源：

POTATO RESEARCH | 1992年 / 35卷 / 04期

关键词：

ALPHA-CHACONINE; ALPHA-SOLAMARGINE; ALPHA-SOLANINE; ALPHA-SOLASONINE; SOLANUM; POTATO;

D O I：

10.1007/BF02357601

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Methods were developed for clean-up of potato tuber extracts on solid phase extraction CN-cartridges and for analysis of steroidal glycoalkaloids by reversed phase HPLC. The alkaloids alpha-solanine, alpha-chaconine, alpha-solasonine and alpha-solamargine could be separated on C-8 or C18 reversed phase columns using a mobile phase of acetonitrile/Tris-buffer (3:2, v/v). The analysis time of tuber extracts containing these alkaloids was less then 4 min, if the pH of the mobile phase was adjusted to 7.4 and run at a flow rate of 0.5 ml/min.

引用

页码：451 / 455

页数：5

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