THE UNFOLDING AND ATTEMPTED REFOLDING OF CITRATE SYNTHASE FROM PIG-HEART

被引：38

作者：

WEST, SM ^{[1
]}

KELLY, SM ^{[1
]}

PRICE, NC ^{[1
]}

机构：

[1] UNIV STIRLING,SCH MOLEC & BIOL SCI,STIRLING FK9 4LA,SCOTLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1037卷 / 03期

关键词：

Circular dichroism; Citrate synthase; Mitochondrial protein assembly; Protein folding; Protein unfolding;

D O I：

10.1016/0167-4838(90)90034-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The unfolding of the dimeric enzyme citrate synthase from pig heart in solutions of guanidinium chloride (GdnHCl) was studied. Data from fluorescence, circular dichroism (CD) and thiol group reactivity studies indicated that the enzyme was almost completely unfolded at GdnHCl concentrations ≥ 4 M. On dilution of GdnHCl, essentially no reactivation of the enzyme occurred. The implications of this finding for the process of folding and assembly in vivo of this and other mitochondrial enzymes are discussed. Exposure of the enzyme to high pH (9-10) led to only a small loss of secondary structure and partial reactivation could be observed on readjustment of the pH to 8.0. © 1990.

引用

页码：332 / 336

页数：5

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