CLINICAL-EVALUATION OF A NEW INVITRO ASSAY FOR SPECIFIC IGE, THE IMMUNO CAP SYSTEM

被引：82

作者：

LEIMGRUBER, A ^{[1
]}

MOSIMANN, B ^{[1
]}

CLAEYS, M ^{[1
]}

SEPPEY, M ^{[1
]}

JACCARD, Y ^{[1
]}

AUBERT, V ^{[1
]}

PEITREQUIN, R ^{[1
]}

NISOLI, MP ^{[1
]}

PECOUD, A ^{[1
]}

机构：

[1] CHU VAUDOIS,DIV IMMUNOL & ALLERGY,CH-1011 LAUSANNE,SWITZERLAND

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1991年 / 21卷 / 01期

关键词：

D O I：

10.1111/j.1365-2222.1991.tb00814.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

The Pharmacia CAP system is a new assay for specific IgE characterized by a new solid phase (hydrophilic polymer encased in a capsule). The CAP results were compared to those of the Phadebas IgE RAST and skin-prick tests (SPT) performed in 145 subjects suffering from rhinitis and/or asthma with eight common inhalant allergens (total number of tests = 1160). Concording CAP/RAST results were found in 91% of the tests. The CAP was positive in 78% and the RAST in 65% of the positive SPT. Conversely, the CAP was negative in 90.6% and the RAST in 96.4% of the negative SPT. A pattern 'negative SPT, negative RAST and positive CAP' was found in 56 tests (40 subjects): in four such subjects, the CAP positivity was confirmed by a positive IgE crossed radioimmunoelectrophoresis. Three borderline positive results were found among 240 negative controls (serum from cord blood or non-atopics). These data indicate that compared with SPT the CAP system is (a) more sensitive than the Pharmacia RAST and (b) does not seem less specific.

引用

页码：127 / 131

页数：5

共 20 条

[1]

ANDERSON MC, 1981, J IMMUNOL, V127, P972

[2]

AXEN RE, 1987, Patent No. 4708932

[3]

AXEN RE, 1988, NEW ENG REG ALLERGY, V9, P503

[4]

BAZARAL M, 1971, J IMMUNOL, V107, P794

[5] ALLERGY DIAGNOSIS WITH RADIOALLERGOSORBENT TEST - COMPARISON WITH RESULTS OF SKIN AND PROVOCATION TESTS IN AN UNSELECTED GROUP OF CHILDREN WITH ASTHMA AND HAY-FEVER [J].

BERG, TLO ;

JOHANSSON, SG .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1974, 54 (04) :209-221

[6]

BERNSTEIN IL, 1988, J ALLERGY CLIN IM S3, V82, P499

[7]

BJORKMAN R, 1988, Patent No. 143097

[8]

BROWN CR, 1985, CLIN CHEM, V31, P1500

[9]

ENGVALL E, 1972, J IMMUNOL, V109, P129

[10] DIAGNOSIS OF REAGINIC ALLERGY WITH HOUSE DUST, ANIMAL DANDER AND POLLEN ALLERGENS IN ADULT PATIENTS .1. COMPARISON BETWEEN RAST, SKIN-TESTS AND PROVOCATION TESTS [J].

ERIKSSON, NE ;

AHLSTEDT, S ;

BELIN, L .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1976, 52 (1-4) :335-346

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