CHROMOGRANIN-A IMMUNOREACTIVITY AND GRIMELIUS-ARGYROPHILIA - A CORRELATIVE STUDY IN MAMMALIAN ENDOCRINE-CELLS

被引:24
作者
CETIN, Y
机构
[1] Abteilung Anatomie I, Medizinische Hochschule Hannover, Hannover 61, W-3000
来源
ANATOMY AND EMBRYOLOGY | 1992年 / 185卷 / 03期
关键词
CHROMOGRANIN-A; GRIMELIUS-ARGYROPHILIA; ENDOCRINE CELLS; IMMUNOHISTOCHEMISTRY;
D O I
10.1007/BF00211819
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Various endocrine cells can be stained by the argyrophil reaction of Grimelius. This silver stain has recently been attributed to chromogranin A, an acidic glycoprotein, that is present in many endocrine cells. Using serial sections of plastic-embedded tissues (adrenal medulla, pancreas, gastric mucosa) various endocrine cells were investigated for their content of chromogranin A immunoreactivity and for their argyrophilia. The findings in four species (man, cattle, pig, guinea pig) showed that chromogranin A immunoreactivity and argyrophil stain partly overlap in identical endocrine cells, but do not necessarily coincide in the majority of endocrine cells. We found that endocrine cells could be positive for chromogranin A and argyrophilia (e.g., aminergic endocrine cells); or positive for chromogranin A but negative for argyrophilia (e.g., insulin cells of all species; somatostatin cells of cattle and pig); or negative for chromogranin A but positive for argyrophilia (e.g., glucagon cells of pig and guinea pig); or negative for chromogranin A and argyrophilia (e.g., somatostatin cells of man and guinea pig). Such heterogeneities of the staining pattern for chromogranin A and argyrophil silver reaction were also observed in individual endocrine cells of a given population (e.g., gastrin cells). Hence, although recent dot-blot tests have shown that chromogranin A is an argyrophilic substance, in tissue sections chromogranin A immunostaining and Grimelius' silver staining did not coincide in various endocrine cells, for unknown reasons. Therefore, it is recommended to use both chromogranin A immunohistochemistry and the classical Grimelius' silver stain to "mark" the vast majority of endocrine cells in tissue sections.
引用
收藏
页码:207 / 215
页数:9
相关论文
共 43 条
[1]   SECRETION OF A CHROMAFFIN GRANULE PROTEIN CHROMOGRANIN FROM ADRENAL GLAND AFTER SPLANCHNIC STIMULATION [J].
BLASCHKO, H ;
COMLINE, RS ;
SCHNEIDER, FH ;
SILVER, M ;
SMITH, AD .
NATURE, 1967, 215 (5096) :58-+
[2]  
BUSSOLATI G, 1985, AM J PATHOL, V120, P186
[3]  
CETIN Y, 1990, HISTOCHEMISTRY, V94, P31
[4]   IMMUNOREACTIVITIES FOR CHROMOGRANIN-A AND CHROMOGRANIN-B, AND SECRETOGRANIN-II IN THE GUINEA-PIG ENDOCRINE PANCREAS [J].
CETIN, Y ;
GRUBE, D .
HISTOCHEMISTRY, 1990, 94 (05) :479-484
[5]   IMMUNOREACTIVITIES FOR CHROMOGRANIN-A AND CHROMOGRANIN-B, AND SECRETOGRANIN-II IN THE GUINEA-PIG ENTERO-ENDOCRINE SYSTEM - CELLULAR-DISTRIBUTIONS AND INTERCELLULAR HETEROGENEITIES [J].
CETIN, Y ;
GRUBE, D .
CELL AND TISSUE RESEARCH, 1991, 264 (02) :231-241
[6]   CHROMOGRANIN-A (CGA) IN THE GASTROENTEROPANCREATIC (GEP) ENDOCRINE SYSTEM .2. CGA IN MAMMALIAN ENTERO-ENDOCRINE CELLS [J].
CETIN, Y ;
MULLERKOPPEL, L ;
AUNIS, D ;
BADER, MF ;
GRUBE, D .
HISTOCHEMISTRY, 1989, 92 (04) :265-275
[7]   CHROMOGRANIN-A IN THE PANCREATIC-ISLET - CELLULAR AND SUBCELLULAR-DISTRIBUTION [J].
EHRHART, M ;
GRUBE, D ;
BADER, MF ;
AUNIS, D ;
GRATZL, M .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1986, 34 (12) :1673-1682
[8]   FLUORESCENCE OF CATECHOL AMINES AND RELATED COMPOUNDS CONDENSED WITH FORMALDEHYDE [J].
FALCK, B ;
THIEME, G ;
HILLARP, NA ;
TORP, A .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1962, 10 (03) :348-&
[9]   DOPAMINE BETA-HYDROXYLASE AND OTHER GLYCOPROTEINS FROM THE SOLUBLE CONTENT AND THE MEMBRANES OF ADRENAL CHROMAFFIN GRANULES - ISOLATION AND CARBOHYDRATE ANALYSIS [J].
FISCHERCOLBRIE, R ;
SCHACHINGER, M ;
ZANGERLE, R ;
WINKLER, H .
JOURNAL OF NEUROCHEMISTRY, 1982, 38 (03) :725-732
[10]   A NEW METHOD FOR DEMONSTRATING ARGYROPHIL CELLS OF THE PANCREAS AND INTESTINES [J].
GARVEY, W ;
FATHI, A ;
BIGELOW, F ;
CARPENTER, B ;
JIMENEZ, C .
STAIN TECHNOLOGY, 1989, 64 (02) :87-91