INVOLVEMENT OF PHOSPHOINOSITIDE TURNOVER IN OUABAIN INOTROPISM

被引：10

作者：

GOTOH, H

KAMIYAMA, A

SHIBAYAMA, R

SAWADA, M

KASHIMOTO, T

机构：

[1] IWATE UNIV,DEPT PHARMACOL,MORIOKA,IWATE 020,JAPAN

[2] TEIKYO UNIV,SCH MED,DEPT PHYSIOL,ITABASHI KU,TOKYO 173,JAPAN

[3] ONO PHARMACEUT CO LTD,RES INST,MISHIMA,OSAKA 618,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined whether ouabain activates phospholipases and reinforces contraction force of papillary muscles through resultant second messengers. 2-Nitro-4-carboxyphenyl-N, N-diphenylcarbamate (NCDC), the inhibitor of phospholipase C (PLC), abolished the ouabain inotropy in rabbit papillary muscles. Calphostin C, the specific inhibitor of protein kinase C (PKC), also depressed the ouabain inotropy. 12-O-Tetradecanoylphorbor-13-acetate (TPA), the specific activator of PKC, enhanced the beat-to-beat phasic contractility at low concentrations. Radioenzymatic assay revealed that ouabain treatment doubled diacylglycerol (DG) content in excised papillary muscles. We concluded that ouabain activates PLC, and the resultant second messenger, DG, augments the cardiac contraction force through activation of PKC. © 1993 Academic Press, Inc.

引用

页码：72 / 78

页数：7

共 23 条

[1] NA+-K+-ATPASE INHIBITION STIMULATES PGE RELEASE IN GUINEA-PIG TAENIA-COLI [J].