MICROTUBULE ORGANIZATION IN THE COW DURING FERTILIZATION, POLYSPERMY, PARTHENOGENESIS, AND NUCLEAR TRANSFER - THE ROLE OF THE SPERM ASTER

被引:193
作者
NAVARA, CS
FIRST, NL
SCHATTEN, G
机构
[1] UNIV WISCONSIN,DEPT MEAT & ANIM SCI,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT ZOOL,MADISON,WI 53706
关键词
D O I
10.1006/dbio.1994.1064
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Microtubule organization in bovine oocytes during fertilization, polyspermy, parthenogenesis, and nuclear transfer is examined with the goal of understanding microtubule activity and the manner in which the centrosome during fertilization and mitotic spindle poles are established. In the unfertilized bovine oocyte, microtubules are detected only in the metaphase-arrested second meiotic spindle; no cytoplasmic asters are observed. After insemination, a small aster of microtubules is seen adjacent to the incorporated sperm head. This aster enlarges and, at the time of pronuclear apposition, fills the cytoplasm. At prophase, the aster splits and forms the poles for the first mitotic spindle, which is anastral, fusiform, and often located eccentrically. During anaphase, asters assemble at each spindle pole, After telophase, these asters develop into the interphase array of microtubules in the daughter blastomeres. During polyspermy, an aster forms from a site between each incorporated sperm head and tail. Multiple mitotic spindles are observed in polyspermic zygotes; multipolar spindles are not seen. Parthenogenetic activation with 5 mu m ionomycin followed by a 4-hr incubation in 1.9 mM dimethylaminopurine results in >80% activation, and antitubulin immunofluorescence microscopy demonstrates that initially disarrayed microtubules are observed and the some microtubules extend from the remnants of the second meiotic spindle. At the time normal for cell division, these parthenogenotes form anastral, barrel-shaped bipolar mitotic spindies. Asters form at the spindle poles at anaphase and the parthenogenotes divide from one to two blastomeres. Nuclear fusion of a morula-derived blastomere to a parthenogenetically activated oocyte results in either a single microtubule aster or at times, two asters, formed in association with the donated nucleus. These results demonstrate that fertilization in the cow is a blending of paternally and maternally derived centrosomal material. (C) 1994 Academic Press, Inc.
引用
收藏
页码:29 / 40
页数:12
相关论文
共 42 条
[1]   FORMATION OF THE 1ST CLEAVAGE SPINDLE IN NEMATODE EMBRYOS [J].
ALBERTSON, DG .
DEVELOPMENTAL BIOLOGY, 1984, 101 (01) :61-72
[2]   DEVELOPMENT OF PRE-IMPLANTATION EMBRYOS OF THE GOLDEN-HAMSTER IN A DEFINED CULTURE-MEDIUM [J].
BAVISTER, BD ;
LEIBFRIED, ML ;
LIEBERMAN, G .
BIOLOGY OF REPRODUCTION, 1983, 28 (01) :235-247
[3]  
Boveri T., 1901, ZELLEN STUDIEN NATUR
[4]  
BREED WG, 1994, IN PRESS DEV BIOL
[5]   CENTROSOME DEVELOPMENT IN EARLY MOUSE EMBRYOS AS DEFINED BY AN AUTOANTIBODY AGAINST PERICENTRIOLAR MATERIAL [J].
CALARCOGILLAM, PD ;
SIEBERT, MC ;
HUBBLE, R ;
MITCHISON, T ;
KIRSCHNER, M .
CELL, 1983, 35 (03) :621-629
[6]   FERTILIZATION AND EARLY DEVELOPMENT IN BEROE-OVATA [J].
CARRE, D ;
SARDET, C .
DEVELOPMENTAL BIOLOGY, 1984, 105 (01) :188-195
[7]  
CHU D, 1987, 1ST INT S CYT DEV, V8, P331
[8]   REGION-SPECIFIC CELL ACTIVITIES IN AMPHIBIAN GASTRULATION [J].
GERHART, J ;
KELLER, R .
ANNUAL REVIEW OF CELL BIOLOGY, 1986, 2 :201-229
[9]  
GUETHHALLONET C, 1993, J CELL SCI, V105, P157
[10]   SPINDLE POLE CENTROSOMES OF SEA-URCHIN EMBRYOS ARE PARTIALLY COMPOSED OF MATERIAL RECRUITED FROM MATERNAL STORES [J].
HOLY, J ;
SCHATTEN, G .
DEVELOPMENTAL BIOLOGY, 1991, 147 (02) :343-353