HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHODS FOR STUDYING PROTEIN-BINDING OF ALUMINUM IN HUMAN SERUM IN THE ABSENCE AND IN THE PRESENCE OF DESFERRIOXAMINE

被引：33

作者：

ALONSO, JIG

GARCIA, AL

PARAJON, JP

GONZALEZ, EB

MEDEL, AS

CANNATA, JB

机构：

[1] UNIV OVIEDO,DEPT PHYS & ANALYT CHEM,C JULIA CLAVERIA S-N,E-33006 OVIEDO,SPAIN

[2] HOSP GEN ASTURIAS,RES UNIT,OVIEDO,SPAIN

来源：

CLINICA CHIMICA ACTA | 1990年 / 189卷 / 01期

关键词：

Aluminium; Desferrioxamine; High performance liquid chromatography; Protein binding; Transferrin;

D O I：

10.1016/0009-8981(90)90236-L

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Ion-exchange high performance liquid chromatography of serum proteins was combined with aluminium determination by electrothermal-atomisation-atomic-absorption spectroscopy and fluorimetry for studying the distribution of aluminium in human serum in the absence and in the presence of desferrioxamine. Aluminium was eluted as a single peak in the same fraction as transferrin. However, following the addition of desferrioxamine most of the aluminium was liberated from transferrin and become attached to the chelator. © 1990.

引用

页码：69 / 80

页数：12

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