DEPENDENCE OF 3-D OPTICAL TRANSFER-FUNCTIONS ON THE PINHOLE RADIUS IN A FLUORESCENT CONFOCAL OPTICAL MICROSCOPE

被引：23

作者：

KIMURA, S

MUNAKATA, C

机构：

[1] Hitachi Ltd., Central Research Laboratory, Kokubunji, Tokyo

来源：

APPLIED OPTICS | 1990年 / 29卷 / 20期

关键词：

D O I：

10.1364/AO.29.003007

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The resolution of a fluorescent confocal scanning optical microscope (CSOM) is superior to that of a conventional fluorescent optical microscope. To attain this superiority, the fluorescent CSOM uses a pinhole in front of the detector. Thus, the resolution of the CSOM is dependent on the pinhole radius. Threedimensional optical transfer functions are calculated for the various radii to elucidate this dependence. The results show that a CSOM with a radius smaller than ˜1 optical unit has a bandwidth comparable with that of an infinitely small radius. © 1990 Optical Society of America.

引用

页码：3007 / 3011

页数：5

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