REMARKABLE CHANGES IN CATALYTIC ACTIVITY TOWARD TESTOSTERONE OF ENGINEERED CYTOCHROME P-450D BY MUTATIONS AT PUTATIVE DISTAL SITE

被引：5

作者：

HIROYA, K ^{[1
]}

SHIMIZU, T ^{[1
]}

HATANO, M ^{[1
]}

FUJIIKURIYAMA, Y ^{[1
]}

机构：

[1] TOHOKU UNIV, FAC SCI, DEPT CHEM, AOBA KU, SENDAI, MIYAGI 980, JAPAN

来源：

CHEMISTRY LETTERS | 1991年 / 06期

关键词：

D O I：

10.1246/cl.1991.973

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The catalytic activity, 6-beta-hydroxylation, toward testosterone of engineered cytochrome P-450d was three-times increased by Thr319Ala mutation at the putative distal site, while that was abolished by mutations Ala315Ser and Thr322Ala at the same site, suggesting the important role of this region in catalytic activity toward the steroid of cytochrome P-450d.

引用

页码：973 / 976

页数：4

共 23 条

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