DETERMINATION OF GALACTOMANNAN (GUM) IN GUAR (CYAMOPSIS-TETRAGONOLOBUS) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：13

作者：

HANSEN, RW ^{[1
]}

BYRNES, SM ^{[1
]}

JOHNSON, AD ^{[1
]}

机构：

[1] GRIFFITH UNIV,SCH AUSTRALIAN ENVIRONM STUDIES,NATHAN,QLD 4111,AUSTRALIA

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1992年 / 59卷 / 03期

关键词：

GUAR; CYAMOPSIS-TETRAGONOLOBUS; GALACTOMANNAN; GUM; SUGARS; HPLC;

D O I：

10.1002/jsfa.2740590322

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A method is described for analysis by HPLC of galactomannan (gum) in seed of guar (Cyamopsis tetragonolobus (L) Taub). The dry seed is ground in 950 ml litre-1 ethanol, and free sugars are removed by ethanol extract; the seed residue is hydrolysed with 2 M trifluoracetic acid in a pressure cooker (1 h at approximately 105 kPa). Hydrolysing the gum in situ avoids the problems common with other techniques of extracting different fractions of gum, each having different mannose: galactose ratios. The method serves as a simple measure of gum contents in guar, and up to 20 samples per day can be processed.

引用

页码：419 / 421

页数：3

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