SEMIAUTOMATED DETECTION OF ABERRANT CHROMOSOMES IN BIVARIATE FLOW KARYOTYPES

被引：15

作者：

BOSCHMAN, GA ^{[1
]}

MANDERS, EMM ^{[1
]}

RENS, W ^{[1
]}

SLATER, R ^{[1
]}

ATEN, JA ^{[1
]}

机构：

[1] UNIV AMSTERDAM,INST HUMAN GENET,1105 AZ AMSTERDAM,NETHERLANDS

来源：

CYTOMETRY | 1992年 / 13卷 / 05期

关键词：

FLOW CYTOMETRY; HUMAN CHROMOSOMES; NEOPLASIA; KARYOTYPE; CHROMOSOME ABNORMALITIES;

D O I：

10.1002/cyto.990130504

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described that is designed to compare, in a standardized procedure, bivariate flow karyotypes of Hoechst 33258 (HO)/ Chromomycin A3 (CA) stained human chromosomes from cells with aberrations with a reference flow karyotype of normal chromosomes. In addition to uniform normalization of normal and abnormal flow karyotypes, the main purpose is detection of structurally abnormal chromosomes in often complex karyotypes of tumor cells. The method, which has been implemented in a computer program, consists of a comparison of individual chromosome peaks with the positions of peaks in the flow karyotype constituted by normal chromosomes and takes into account the natural variability in base composition of normal chromosomes among healthy individuals. Flow-karyotypes are normalized using an iterative fitting procedure, using corrections for (1) amplification of HO and CA fluorescence, (2) cross-talk between the fluorescence signals of HO and CA, and (3) offset of the HO and CA origin. Flow karyotypes of two cell lines, one with a simple deletion and the other with more complex karyotypic changes, were analyzed. The results of flow analysis were found to be in general agreement with the cytogenetic analysis of quinacrine banded karyotypes.

引用

页码：469 / 477

页数：9

共 29 条

[1] ARKESTEIJN GJA, 1988, BLOOD, V72, P282
[2] CLINICAL-APPLICATIONS OF FLOW KARYOTYPING IN MYELOCYTIC-LEUKEMIA BY STIMULATION OF DIFFERENT SUBPOPULATIONS OF CELLS IN BLOOD OR BONE-MARROW SAMPLES
ARKESTEIJN, GJA
VANDEKKEN, H
MARTENS, ACM
HAGENBEEK, A
[J]. CYTOMETRY, 1990, 11 (01): : 196 - 201
[3] BIVARIATE FLOW KARYOTYPING OF HUMAN-CHROMOSOMES - EVALUATION OF VARIATION IN HOECHST-33258 FLUORESCENCE, CHROMOMYCIN-A3 FLUORESCENCE, AND RELATIVE CHROMOSOMAL DNA CONTENT
BOSCHMAN, GA
RENS, W
VANOVEN, CH
MANDERS, EMM
ATEN, JA
[J]. CYTOMETRY, 1991, 12 (06): : 559 - 569
[4] MOLECULAR-BIOLOGY AND GENETICS OF HUMAN NEURO-BLASTOMA
BRODEUR, GM
FONG, CT
[J]. CANCER GENETICS AND CYTOGENETICS, 1989, 41 (02) : 153 - 174
[5] STUDY OF X-CHROMOSOME ABNORMALITY IN XX-MALES USING BIVARIATE FLOW KARYOTYPE ANALYSIS AND FLOW SORTED DOT BLOTS
CARTER, NP
FERGUSONSMITH, ME
AFFARA, NA
BRIGGS, H
FERGUSONSMITH, MA
[J]. CYTOMETRY, 1990, 11 (01): : 202 - 207
[6] ANALYSIS OF BIVARIATE FLOW KARYOTYPES
DEAN, PN
KOLLA, S
VANDILLA, MA
[J]. CYTOMETRY, 1989, 10 (02): : 109 - 123
[7] HIGH-RESOLUTION DUAL LASER FLOW CYTOMETRY
DEAN, PN
PINKEL, D
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1978, 26 (08) : 622 - 627
[8] HIGH-RESOLUTION CHROMOSOME ANALYSIS - ONE AND 2 PARAMETER FLOW CYTOMETRY
GRAY, JW
LANGLOIS, RG
CARRANO, AV
BURKHARTSCHULTE, K
VANDILLA, MA
[J]. CHROMOSOMA, 1979, 73 (01) : 9 - 27
[9] GRAY JW, 1988, AM J HUM GENET, V42, P49
[10] HARNDEN DG, 1985, CYTOGEN CELL GENET

← 1 2 3 →