COMPARISON BETWEEN 2 METHODS TO MEASURE TRANSLATIONAL DIFFUSION OF A SMALL-MOLECULE AT SUBZERO TEMPERATURE

被引:20
作者
CHAMPION, D [1 ]
HERVET, H [1 ]
BLOND, G [1 ]
SIMATOS, D [1 ]
机构
[1] COLL FRANCE,PHYS MAT CONDENSEE LAB,F-75000 PARIS,FRANCE
关键词
SUCROSE SOLUTIONS; FRAP; CONCENTRATION PROFILE; DIFFUSION;
D O I
10.1021/jf00059a022
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The diffusion of a small molecule is one of the determining factors that control evolution in food products during storage at low water content or in the frozen state. The diffusion of a small molecule, fluorescein, in highly viscous media (concentrated sucrose solutions) has been studied, using two tracer techniques: a concentration profile method and fluorescence recovery after photobleaching (FRAP). In the large temperature range studied, from -10 to 20 degrees C, the diffusion coefficients of fluorescein, obtained with the two methods, are similar. FRAP can be used only for transparent samples, but it is a rapid method to determine low diffusion coefficients. The concentration profile method can be used for diffusion experiments in nontransparent samples, containing ice for example.
引用
收藏
页码:2887 / 2891
页数:5
相关论文
共 22 条
[1]  
BETTENFELD ML, 1985, THESIS U DIJON ENSBA
[2]   DIFFUSION OF DEXTRAN IN AQUEOUS (HYDROXYPROPYL)CELLULOSE [J].
BU, Z ;
RUSSO, PS .
MACROMOLECULES, 1994, 27 (05) :1187-1194
[3]   VOLATILES RETENTION DURING DRYING OF FOOD LIQUIDS [J].
CHANDRASEKARAN, SK ;
KING, CJ .
AICHE JOURNAL, 1972, 18 (03) :520-+
[4]  
Crank J., 1979, MATH DIFFUSION
[5]  
DAVOUST J, 1982, EMBO J, V4, P229
[6]   STRUCTURAL INFORMATION ON HYALURONIC-ACID SOLUTIONS AS STUDIED BY PROBE DIFFUSION EXPERIMENTS [J].
DE SMEDT, SC ;
LAUWERS, A ;
DEMEESTER, J ;
ENGELBORGHS, Y ;
DEMEY, G ;
DU, M .
MACROMOLECULES, 1994, 27 (01) :141-146
[7]  
FRICK TS, 1989, THESIS U MINNESOTA
[8]  
HAGLUND BO, 1988, CHEM SCRIPTA, V28, P129
[9]  
Hemminga M. A., 1992, Trends in Food Science & Technology, V3, P179, DOI 10.1016/0924-2244(92)90186-Z
[10]   MASS DIFFUSION MEASUREMENTS IN LIQUID-CRYSTALS BY A NOVEL OPTICAL METHOD [J].
HERVET, H ;
URBACH, W ;
RONDELEZ, F .
JOURNAL OF CHEMICAL PHYSICS, 1978, 68 (06) :2725-2729