A NOVEL GAMMA-SUBUNIT OF THE GABA-A RECEPTOR IDENTIFIED USING THE POLYMERASE CHAIN-REACTION

被引：73

作者：

WILSONSHAW, D

ROBINSON, M

GAMBARANA, C

SIEGEL, RE

SIKELA, JM

机构：

[1] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,C-236,4200 E 9TH AVE,DENVER,CO 80262

[2] CASE WESTERN RESERVE UNIV,DEPT PHARMACOL,CLEVELAND,OH 44106

来源：

FEBS LETTERS | 1991年 / 284卷 / 02期

关键词：

GABA-A RECEPTOR; LIGAND-GATED ION CHANNEL; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(91)80687-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, gamma-3, of the GABAA receptor. The gamma-3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABA(A) receptor family. The gamma-3 subunit shares approximately 66% sequence identity with the gamma-2 subunit but only 38% and 29% with alpha-1 and beta-1 subunits, respectively. Localization of the gamma-3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the gamma-2 subunits.

引用

页码：211 / 215

页数：5

共 23 条

[1] ALCOHOL-INTOXICATION - ION CHANNELS AND GENETICS [J].