MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE DETERMINATION OF THE REGULATOR REGION OF MECA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS (MRSA)

被引：145

作者：

HIRAMATSU, K ^{[1
]}

ASADA, K ^{[1
]}

SUZUKI, E ^{[1
]}

OKONOGI, K ^{[1
]}

YOKOTA, T ^{[1
]}

机构：

[1] TAKEDA CHEM IND LTD,DIV CENT RES,YODOGAWA KU,OSAKA 532,JAPAN

来源：

FEBS LETTERS | 1992年 / 298卷 / 2-3期

关键词：

MECA; REPRESSOR; CO-INDUCER; METHICILLIN RESISTANCE; STAPHYLOCOCCUS-AUREUS;

D O I：

10.1016/0014-5793(92)80039-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular cloning and nucleotide sequence analysis were performed for the identification of the regulator genes of methicillin resistance in the genome of a MRSA strain N315. Two open reading frames (orfs) were identified in the 5'-flanking region of the mecA gene. Predicted amino acid sequences of these orfs showed extensive homology to the co-inducer and the repressor protein of the penicillinase (PCase) production in Staphylococcus aureus as well as in Bacillus licheniformis. These orfs are considered to encode putative co-inducer and repressor proteins specific for the regulation of methicillin resistance in MRSA.

引用

页码：133 / 136

页数：4

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